In vitro efficacy assays based on secreted biomarkers
SecretCells is formed by the reversible assembly of a biochip to a glass based biosensor. Cells are grown in either 2D or 3D, depending on the experimental setup, under a controlled perfusion of nutrients and compounds to be tested. Specific protein probes are grafted on the biosensor surface to create a microarray for the capture of secreted biomarkers. Biomarkers secretion is evaluated after incubation with specific reagents (ELISA sandwich) by imaging the biosensor surface for fluorescent signals.
Microfluidics
Microfluidic systems offer many advantages:
- Increased precision
- Reduced consumption of costly reagents
- Improved experimental reproducibility
- Realization of different steps of an experiment on a single device
Biosensors
Nanobiose develops biosensors dedicated to multiplexed analysis of secreted biomarkers.
- Fluorescence based readout
- Adaptable on fluorescence microscopes and slide scanners
- Customization (species, biomarkers) upon request
Real-time capture of secreted biomarkers
Real-time provides access to critical information to understand biological processes, without multiplying the number of samples or assays.
Real-time capture of biomarkers on the biosensor allows to :
- Capture all the time frame of the experiment
- Avoid / reduce loss of biomarkers by degradation
- No handling and intermediate storage of samples
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