In vitro efficacy assays based on secreted biomarkers
SecretCells is formed by the reversible assembly of a biochip to a glass based biosensor. Cells are grown in either 2D or 3D, depending on the experimental setup, under a controlled perfusion of nutrients and compounds to be tested. Specific protein probes are grafted on the biosensor surface to create a microarray for the capture of secreted biomarkers. Biomarkers secretion is evaluated after incubation with specific reagents (ELISA sandwich) by imaging the biosensor surface for fluorescent signals.